Penicillin–streptomycin (double antibiotics) is the most commonly used antibiotic combination in in vitro culture to prevent microbial contamination. Penicillin interferes with the synthesis of bacterial cell walls and is particularly effective against Gram-positive bacteria. Streptomycin binds to the 30S subunit of bacterial ribosomes, inhibiting protein synthesis, and is effective against both Gram-negative and Gram-positive bacteria, especially Gram-negative bacteria. The combined use of penicillin and streptomycin can prevent most bacterial contamination.
English
KR
IA
JP

