PBMC Separation Tubes

Phone Number

+91 9108788989

Email

contact@biostarlifetech.com

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Specification: 15 mL/50 mL with separation bracket
Cap Type: Flat
Bottom Type: Conical
Separation Bracket Type: Eight-hole Cylindrical

Product Information

The JET BIOFIL PBMC Separation Tubes boast a unique design featuring a built-in separation bracket, which effectively reduce the mixing of target samples and the density gradient medium during the centrifugation process. This design allows Mononuclear Cells (MNCs) to be retained above the separation bracket, separating them from the red blood cells and granulocyte layer present at the tube’s bottom. MNCs can be effortlessly collected without the need for complex steps, streamlining experiments and saving valuable time compared to traditional methods.

The PBMC Separation Tube strictly adheres to the Good Manufacturing Practice (GMP) standards. It meets the requirements for biological laboratory consumables with a higher cleanliness grade, ensuring its suitability for various experimental applications.

PBMC Separation Tubes Features

Features

The built-in separation bracket minimizes the mixing of the sample and the separation medium, thereby avoiding the need for slow and laborious application of the sample to the upper layer of the density gradient medium

Easy to operate, with MNCs collected by directly pouring out after centrifugation

High consistency minimizes the influence of human operation on experimental outcomes

Rapidly isolates peripheral blood mononuclear cells (PBMCs) within a 15-minute time frame

Features

The PBMC Separation Tube is manufactured in strict adherence to GMP standards, and the finished items undergo rigorous third-party testing to meet the experimental requirements for consumables with a higher cleanliness grade

Triple independent bagged clean medical outer packaging, with product lot number marked on the innermost layer for traceability

Sterilized by irradiation, SAL 10-6

DNase/RNase-free, non-pyrogenic

Material

Tube Body

Polypropylene (PP)

Tube Cap

High-density polyethylene (HDPE)

Separation Bracket

Methyl methacrylate-butadiene-styrene (MBS)

Conforming to USP Class VI Standards

Order Information

PBMC Separation Tubes

Cat. No.	Description	Sterile	Undiluted Volume	Qty. Per Bag	Qty. Per Case
CSP021015	Tube with separation bracket (15 mL/tube)	Y	0.5–4 mL	25	100
CSP021050	Tube with separation bracket (50 mL/tube)	Y	4–17 mL	25	100

Frequently Asked Questions

What is the maximum rotational speed that the centrifuge tubes of Jet Biofil can withstand?

21000xg. Due to the different center distance of the rotor shaft of different centrifuges, the centrifugal force generated at the same speed (rpm) is also different, so we usually use the relative centrifugal force (RCF) (g) to represent the tolerance degree of the centrifuge tube in the process of rotating centrifugation.

Can the centrifuge tubes of Jet Biofil hold some kind of solution? How about its chemical compatibility?

It is necessary to pay attention to whether the sample is compatible with the material of the centrifuge tube as is used, especially if the sample is some chemical reagents or organic solvents, and the Chemical Compatibility Table of PP Plastic Materials can be used as reference. Samples with low or incompatible centrifugal compatibility are not recommended, and customers can also do pre-experiments to have a test.

What is the possible reason for the burst of the centrifuge tubes in the process of centrifuge?

When centrifuging, it is necessary to pay attention to the chosen centrifugal force cannot exceed the maximum centrifugal force that the product itself can withstand.

Please notice that the maximum centrifugal force (RCF) that can be withstood may be decreased when the sample is centrifuged at low temperature or the sample is an organic solvent/volatile irritant

What should be pay attention to when using the centrifuge tubes to preserve the samples at a low temperature?

When the sample is preserved below 0 ℃, the applicability of the centrifuge tube is closely related to the composition of the solution and preservation conditions. The centrifuge tube cannot be placed on the foam support, nor the bottom of the tube is in contact with the thermal insulation materials. And please use a hollow support when preserving the samples at low temperature. Otherwise, as part of the centrifuge tube is protected by thermal insulation material, while the other part is not, the temperature difference of the overall tube body makes the tube prone to burst. In addition, please reserve a space of at least 10% of the total volume to avoid sample spillage or tube burst caused by volume expansion due to liquid solidification.

Why the centrifuge tube smell special when its package is opened?

If you smell some odor in the product you receive, it is not because of the abnormal quality of the product. This is because some molecules will be vaporized and retained in the product/packaging in the process of high temperature injection molding/irradiation sterilization of the raw material plastic particles of centrifuge tube products.

The materials used for making the centrifuge tubes of Jet Biofil comply to the USP Class VI standards (USP Class VI Chapter, "Biological reactivity Tests, in Vitro" and Chapter, "Biological Reactivity Tests, in vivo”). No cytolysis or cytotoxic effects were observed through the verification. And the outgoing products in the process of quality inspection have passed various verification tests and are in line with the product release standards.