Plasmid Maxiprep Purification Column

Phone Number

+91 9108788989

Email

contact@biostarlifetech.com

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Plasmid Maxiprep Purification Column

Filter Membrane Layers: 6 layers

Purification Column Volume: 20 mL

Centrifuge Tube Volume: 50 mL

Product Information

Plasmid Maxiprep Purification Column is primarily used for the extraction and purification of plasmid DNA, widely applied in genetic engineering and molecular biology research. Jet Biofil’s Plasmid Purification Column is made from medical-grade, six-layer glass fiber membrane, ensuring stable performance, high binding capacity, and strong impact resistance. It can be used with kits for large-scale plasmid extraction, quickly preparing over 1000 µg of plasmid DNA. The product is suitable for applications such as enzyme digestion, transformation, PCR amplification, sequencing, and library construction

Plasmid Maxiprep Purification Column Features

Features

Injection molded purification column results a robust structure. The bottom grid, combined with a compression ring, securely holds the filter membrane in place, preventing breakage during centrifugation, and supporting a maximum RCF of 6,000×g

1µm large-pore filter membrane offers strong permeability and prevents column clogging

Handling up to 20 mL of sample in a single run, quickly yielding more than 1000 µg of plasmid DNA

Features

Tube cap design includes an internal O-ring as gasket, ensuring high sealing integrity to prevent sample leakage

Clear, accurate black graduations with an accuracy of ±2%, allowing precise operation for lab personnel

Extracting high-purity plasmid DNA, with an OD260/OD280 ratio of 1.8-2.0 and an OD260/OD230 ratio greater than 2.0

Compatible with most standard reagent kits, DNase/RNase-free, non-pyrogenic

Material

Filter membrane

Glass fiber, Purification column body and centrifuge

tube body

polypropylene (PP), Tube cap: high-density polyethylene (HDPE)

O-ring Gasket

Thermoplastic Elastomer (TPE)

Conforming to USP Class VI Standards

Order Information

Plasmid Maxiprep Purification Column

Cat. No.	Description	Tube Volume	Purification column Volume	Membrane Layers	Sterile	Qty./Bag	Qty./Case
NAP006050	Plasmid Maxiprep Purification Column	50 mL	20 mL	6	N	50	200

Frequently Asked Questions

What is the maximum rotational speed that the centrifuge tubes of Jet Biofil can withstand?

21000xg. Due to the different center distance of the rotor shaft of different centrifuges, the centrifugal force generated at the same speed (rpm) is also different, so we usually use the relative centrifugal force (RCF) (g) to represent the tolerance degree of the centrifuge tube in the process of rotating centrifugation.

Can the centrifuge tubes of Jet Biofil hold some kind of solution? How about its chemical compatibility?

It is necessary to pay attention to whether the sample is compatible with the material of the centrifuge tube as is used, especially if the sample is some chemical reagents or organic solvents, and the Chemical Compatibility Table of PP Plastic Materials can be used as reference. Samples with low or incompatible centrifugal compatibility are not recommended, and customers can also do pre-experiments to have a test.

What is the possible reason for the burst of the centrifuge tubes in the process of centrifuge?

When centrifuging, it is necessary to pay attention to the chosen centrifugal force cannot exceed the maximum centrifugal force that the product itself can withstand.

Please notice that the maximum centrifugal force (RCF) that can be withstood may be decreased when the sample is centrifuged at low temperature or the sample is an organic solvent/volatile irritant

What should be pay attention to when using the centrifuge tubes to preserve the samples at a low temperature?

When the sample is preserved below 0 ℃, the applicability of the centrifuge tube is closely related to the composition of the solution and preservation conditions. The centrifuge tube cannot be placed on the foam support, nor the bottom of the tube is in contact with the thermal insulation materials. And please use a hollow support when preserving the samples at low temperature. Otherwise, as part of the centrifuge tube is protected by thermal insulation material, while the other part is not, the temperature difference of the overall tube body makes the tube prone to burst. In addition, please reserve a space of at least 10% of the total volume to avoid sample spillage or tube burst caused by volume expansion due to liquid solidification.

Why the centrifuge tube smell special when its package is opened?

If you smell some odor in the product you receive, it is not because of the abnormal quality of the product. This is because some molecules will be vaporized and retained in the product/packaging in the process of high temperature injection molding/irradiation sterilization of the raw material plastic particles of centrifuge tube products.

The materials used for making the centrifuge tubes of Jet Biofil comply to the USP Class VI standards (USP Class VI Chapter, "Biological reactivity Tests, in Vitro" and Chapter, "Biological Reactivity Tests, in vivo”). No cytolysis or cytotoxic effects were observed through the verification. And the outgoing products in the process of quality inspection have passed various verification tests and are in line with the product release standards.