The suspension cells do not have the need for cell adhesion, and generally the culture supplies for untreated surface can be used. TC-treated surface can also culture suspension cells, but according to the state and type of cells, some cells may appear adhesion phenomenon.

You can choose the culture vessel with untreated surface to coat the extracellular matrix by yourself. Of course, in this case, vessels with TC-treated surface can also be used

Cell culture bottles with sealed caps provide a closed cell culture environment. If it is needs to connect with the air in the incubator, it is necessary to slightly loosen the cap after the bottle is put into the incubator to allow air to enter. Similarly, when taking out the bottle, tighten the cap first and then take it out. The cap with the filter membrane itself has the function of breathable, and the filter membrane can prevent microbial contamination. Therefore, the cap of the culture bottle can be directly tightened and put into the incubator during use, but it needs to be noted that the filter membrane on the cap cannot be wet, wet filter membrane cannot ensure the passage of gas.

Taking T25 as an example, it refers to a cell culture bottle with a growth surface area of 25 cm2. According to the type of bottle cap and different culture surfaces, different article numbers correspond to different products.